Sample Exercises

NOTE:Adobe Acrobat Reader is required to view the exercise text available from the links below. Get acrobat readerhere. To perform the experiments required to answer the questions, navigate to StarCellBio at http://starcellbio.mit.edu in a Google Chrome web browser. Click 'Try an Experiment' to use StarCellBio as a guest or sign in to a student account using the course code 'SCB_Sample Exercises'. The assignment text is also available within the 'Assignments' tab in the StarCellBio program.

Answers to all sample exercises are available upon request. Please send an email tostar@mit.eduto request answer keys.

The StarCellBio exercises are available under aCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Exercise 1

• Concept: subcellular localization of proteins
• Experimental technique: microscopy

Exercise 2

• Concept: transmembrane protein orientation
• Experimental techniques: western blotting, flow cytometry

Exercise 3

• Concepts: protein expression levels, subcellular localization, signaling activity, transmembrane protein orientation, and internalization dynamics
• Experimental techniques: western blotting, flow cytometry, microscopy